The hospital-based cholera surveillance was initiated from May, 2014 in 10 hospitals with the collaboration of Institute of Epidemiology, Disease Control and Research (IEDCR; GoB) and icddr,b. This surveillance was further extended in 2016 to 12 more health facilities to have representative data covering hotspots spanning different geographical areas in Bangladesh. However, surveillance sites had been reduced to 16 sites since April 2020. The objective of cholera surveillance in Bangladesh was to develop a laboratory-based surveillance to report culture confirmed cholera cases, describe the co-morbidities, identify disease burden in Bangladesh. Also, to develop and scaling up of a nationwide surveillance system for cholera, surveillance covered different levels of health facilities (Upazila, district and tertiary levels). From June, 2022, surveillance has been continuing with 2 different approaches. In Standard surveillance (12 sites): 20 samples/week/site and in Enhanced surveillance (4 sites): all possible stool samples are being collected by designated trained field attendants (TFAs). Field site Rapid diagnostic test (RDT) is being carried out for all collected samples and preserve/send for culture/PCR to icddr,b/IEDCR laboratory in Dhaka.